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MICA Human ELISA Kit (ab59570)

Abreviews (1)Q&A (2)

Overview

  • Product nameMICA Human ELISA KitSee all MICA kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 6 2214.6pg/ml 50.3 2.3%
    B 6 1102.1pg/ml 39.5 3.6%
    C 6 641.6pg/ml 47.7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    A 18 2416pg/ml 187.1 7.7%
    B 18 1200pg/ml 105.2 8.8%
    C 18 627pg/ml 50.9 8.1%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    123 pg/ml
  • Range
    156 pg/ml - 5000 pg/ml
  • Assay time
    3h 50m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The MICA ELISA is to be used for the in-vitro quantitative determination of MHC class I chain-related gene A glycoprotein, in supernatant,

    buffered solutions, or serum and plasma samples*. This kit has been configured for research use only and is not to be used in diagnostic

    procedures.

    Note: For serum and plasma quandification: high detection level is found in human serum and plasma samples. The high signal appears could be the result of the matrix and/or interaction with other molecules. Consequently serum and plasma quantification is under the scientist responsability.

    Principle

    The MICA Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations and unknown are pipetted into these wells.

    During the first incubation, the MICA antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for MICA is

    incubated. Then the enzyme (streptavidin-horse radish peroxydase) is added. After incubation and washing to remove all unbound enzyme, a

    substrate solution of the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly

    proportional to the concentration of MICA present in the samples.

     

    Range: 156 pg/ml - 5000 pg/ml
    Sensitivity: <123 pg/ml

  • Notes

    Safety

    • For research use only.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash

      thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceThe MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • Cellular localizationPlasma membrane
    • Alternative names
        FLJ60820HLA class I antigen MGC111087
        MHC class I chain related gene A proteinMHC class I chain related protein AMHC class I polypeptide related sequence AMHC class I related proteinMIC A MICAPERB11.1Stress inducible class I homolog
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab59570 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MICA Human ELISA Kit images

      • ELISA - MICA Human ELISA Kit - 2x96 well Plates (ab59570)
        ELISA - MICA Human ELISA Kit - 2x96 well Plates (ab59570)

      References for MICA Human ELISA Kit (ab59570)

      ab59570 has not yet been referenced specifically in any publications.

      Publishing research using ab59570 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab59570 Submit a Question for ab59570

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      Votes: Highest First

      Thanks for your answer. Is these antibodies commercially available as separate/individual products?

      Thank you for your enquiry.

      Unfortunately, we currently do not have these antibodies available in our catalog as individual products.

      However, I am still in contact with our products team and we discuss the options of making them avail...

      Read More

      Dear Abcam,
      Can you please inform me which antibody is used in the MICA ELISA kit ab59570.
      Thanks a lot for you helpJ
      Best Regards,

      Thank you for contacting us.

      The mouse monoclonal antibodies included in this MICA ELISA kit are the following : Clone [B-Z26] is used as Capture, and clone [B-N31] as Detection.

      I hope this information is helpful to you. Please do n...

      Read More

      Abreview for MICA Human ELISA Kit

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews

      Sample: Human Serum (blood of transplanted patients)
      Specification: blood of transplanted patients
      Amount of Sample: 120 µg
      Type of Kit Used: ELISA
      Positive control: Standard supplied by the kit
      Did you use the Abcam Kit protocol?: Yes
      Username

      Verified customer

      Submitted Nov 25 2008

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"