Overview

  • Product name
  • Description
    Rabbit polyclonal to MICALL1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 204-253 (ENGPEEGTFV CAEHCARLGP GTRSGTRPGP FSQPKQQHQQ QLAEDAKDVP) of Human MICALL1 (NP_203744).

  • Positive control
    • Human fetal liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be a cytoskeletal regulator.
  • Sequence similarities
    Contains 1 CH (calponin-homology) domain.
    Contains 1 LIM zinc-binding domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686M2226 antibody
    • FLJ45921 antibody
    • KIAA1668 antibody
    • MICAL-L1 antibody
    • MICAL-like 1 antibody
    • MICAL-like protein 1 antibody
    • Micall1 antibody
    • MILK1_HUMAN antibody
    • MIRab13 antibody
    • Molecule interacting with Rab13 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling MICALL1 with ab105665 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - MICALL1. Right - Merge.


  • Predicted band size : 93 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling MICALL1 with ab105665 at 1.0µg/ml.


  • Predicted band size : 93 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling MICALL1 with ab105665 at 1.0µg/ml.


  • Predicted band size : 93 kDa
    Western blot analysis of HepG2 cell lysate labeling MICALL1 with ab105665 at 1.0µg/ml.


  • Predicted band size : 93 kDa
    Western blot analysis of 293T cell lysate labeling MICALL1 with ab105665 at 1.0µg/ml.
  • Anti-MICALL1 antibody (ab105665) at 0.5 µg/ml + Human fetal liver cell lysate at 10 µg

    Predicted band size : 93 kDa
    Gel concentration: 12%

References

ab105665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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