Overview

  • Product nameAnti-MID1 antibody
    See all MID1 primary antibodies
  • Description
    Mouse monoclonal to MID1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR , corresponding to amino acids 441-540 of Human MID1

Properties

Applications

Our Abpromise guarantee covers the use of ab55444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
    • Tissue specificityIn the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
    • Involvement in diseaseDefects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
    • Sequence similaritiesBelongs to the TRIM/RBCC family.
      Contains 2 B box-type zinc fingers.
      Contains 1 B30.2/SPRY domain.
      Contains 1 COS domain.
      Contains 1 fibronectin type-III domain.
      Contains 1 RING-type zinc finger.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine residues.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • BBBG 1 antibody
      • BBBG1 antibody
      • E3 ubiquitin-protein ligase Midline-1 antibody
      • Finger on X and Y mouse homolog of antibody
      • FXY antibody
      • GBBB 1 antibody
      • GBBB1 antibody
      • MID 1 antibody
      • Mid1 antibody
      • Midin antibody
      • Midline 1 (Opitz/BBB syndrome) antibody
      • Midline 1 antibody
      • Midline 1 ring finger antibody
      • Midline 1 RING finger protein antibody
      • Midline-1 antibody
      • Midline1 antibody
      • OGS 1 antibody
      • OGS1 antibody
      • OS antibody
      • OSX antibody
      • Putative transcription factor XPRF antibody
      • RING finger protein 59 antibody
      • RING finger protein Midline-1 antibody
      • RNF 59 antibody
      • RNF59 antibody
      • TRI18 antibody
      • TRI18_HUMAN antibody
      • TRIM 18 antibody
      • TRIM18 antibody
      • Tripartite motif containing protein 18 antibody
      • Tripartite motif protein TRIM18 antibody
      • Tripartite motif-containing protein 18 antibody
      • XPRF antibody
      • zinc finger on X and Y, mouse, homolog of antibody
      • Zinc finger X and Y antibody
      • ZNFXY antibody
      see all

    Anti-MID1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55444 MID1 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-MID1 antibody (ab55444)

    ab55444 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab55444.
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