Anti-MID1 antibody (ab70770)
Key features and details
- Rabbit polyclonal to MID1
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MID1 antibody -
Description
Rabbit polyclonal to MID1 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide derived from an internal sequence of human MID1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. -
Tissue specificity
In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain. -
Involvement in disease
Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules. -
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 4281 Human
- Entrez Gene: 17318 Mouse
- Entrez Gene: 54252 Rat
- Omim: 300552 Human
- SwissProt: O15344 Human
- SwissProt: O70583 Mouse
- SwissProt: P82458 Rat
- Unigene: 27695 Human
see all -
Alternative names
- BBBG 1 antibody
- BBBG1 antibody
- E3 ubiquitin-protein ligase Midline-1 antibody
see all
Images
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All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution
Lane 1 : 293 extract
Lane 2 : 293 extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa -
ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab70770 has been referenced in 8 publications.
- Ogura Y et al. Mid1 is associated with androgen-dependent axonal vulnerability of motor neurons in spinal and bulbar muscular atrophy. Cell Death Dis 13:601 (2022). PubMed: 35821212
- Chen X et al. Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3. Immunology 163:278-292 (2021). PubMed: 33513265
- Chen Q et al. TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease. Front Physiol 12:709506 (2021). PubMed: 34434118
- Qiao Y et al. MID1 and MID2 regulate cell migration and epithelial-mesenchymal transition via modulating Wnt/ß-catenin signaling. Ann Transl Med 8:1021 (2020). PubMed: 32953821
- Schweiger S et al. Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex. Sci Rep 7:13753 (2017). PubMed: 29062069
- Foong RE et al. Identification of genes differentially regulated by vitamin D deficiency that alter lung pathophysiology and inflammation in allergic airways disease. Am J Physiol Lung Cell Mol Physiol 311:L653-63 (2016). PubMed: 27496895
- Dierssen M et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . PubMed: 22563308
- Li T et al. Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by a4 Protein in HEK293 Cells. Int J Biol Sci 7:740-52 (2011). WB ; Human . PubMed: 21698000