The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Tissue specificityLow level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
DomainThe tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization. Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
Post-translational modificationsPhosphorylated on serine and threonine residues.
ab115604, at 5µg/ml, staining MID2 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-MID2 antibody (ab115604)
Anti-MID2 antibody (ab115604) at 1 µg/ml + Mouse heart lysate at 35 µg Developed using the ECL technique
Predicted band size : 83 kDa
References for Anti-MID2 antibody (ab115604)
has not yet been referenced specifically in any publications.
Publishing research using ab115604? Please let us know so that we can cite the reference in this datasheet.
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