Overview

  • Product nameAnti-MID2 antibody
    See all MID2 primary antibodies
  • Description
    Goat polyclonal to MID2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide:

    QESPYVSGMKTCH

    , corresponding to C terminal amino acids 723-735 of Human MID2 (NP_036348.2).

  • Positive control
    • Human kidney tissue; Mouse heart lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 83 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityLow level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • DomainThe tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
    Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37715 antibody
    • FLJ41813 antibody
    • FXY2 antibody
    • MID2 antibody
    • Midin 2 antibody
    • Midin-2 antibody
    • Midline 2 antibody
    • Midline defect 2 antibody
    • Midline-2 antibody
    • probable E3 ubiquitin protein ligase MID2 antibody
    • Probable E3 ubiquitin-protein ligase MID2 antibody
    • RING finger protein 60 antibody
    • Ring finger protein protein 60 antibody
    • RNF60 antibody
    • TRIM1 antibody
    • TRIM1_HUMAN antibody
    • tripartite motif containing protein 1 antibody
    • Tripartite motif protein 1 antibody
    • Tripartite motif-containing protein 1 antibody
    see all

Anti-MID2 antibody images

  • ab115604, at 5µg/ml, staining MID2 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-MID2 antibody (ab115604) at 1 µg/ml + Mouse heart lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 83 kDa

References for Anti-MID2 antibody (ab115604)

ab115604 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"