Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab14749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with Human MID2 peptide (ab23250).

Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1).

Target

  • Tissue specificity
    Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
    Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37715 antibody
    • FLJ41813 antibody
    • FXY2 antibody
    • MID2 antibody
    • Midin 2 antibody
    • Midin-2 antibody
    • Midline 2 antibody
    • Midline defect 2 antibody
    • Midline-2 antibody
    • probable E3 ubiquitin protein ligase MID2 antibody
    • Probable E3 ubiquitin-protein ligase MID2 antibody
    • RING finger protein 60 antibody
    • Ring finger protein protein 60 antibody
    • RNF60 antibody
    • TRIM1 antibody
    • TRIM1_HUMAN antibody
    • tripartite motif containing protein 1 antibody
    • Tripartite motif protein 1 antibody
    • Tripartite motif-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-MID2 antibody (ab14749) at 0.1 µg/ml

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 81 kDa
  • ab14749 at 5ug/ml staining MID2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

References

ab14749 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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