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Goat polyclonal to MID2
Predicted to work with:
, corresponding to amino acids 703-715 of Human MID2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with
Human MID2 peptide (ab23250).
Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1).
Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
Protein modification; protein ubiquitination.
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
Phosphorylated on serine and threonine residues.
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
Information by UniProt
Anti-MID2 antibody images
References for Anti-MID2 antibody (ab14749)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"