Overview

  • Product nameAnti-Midkine antibody
    See all Midkine primary antibodies
  • Description
    Rabbit polyclonal to Midkine
  • Tested applicationsSuitable for: Indirect ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Midkine

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Midkine.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Midkine (Biotin) (ab83160). Using ab83160 as the detection antibody and ab110614 as the capture antibody, the detection limit for recombinant Human Midkine is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa. The detection limit for recombinant Human Midkine is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionHas heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • Sequence similaritiesBelongs to the pleiotrophin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

References for Anti-Midkine antibody (ab110614)

This product has been referenced in:

See 1 Publication for this product

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The mouse and human proteins are 87% homologues so it is more likely that the antibody will cross react however I can not confirm it, due to non availability of tested data. Please be also aware that thi...

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