Overview

  • Product name
    Anti-Midkine antibody (Biotin)
    See all Midkine primary antibodies
  • Description
    Rabbit polyclonal to Midkine (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Midkine

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83160 is sterile filtered.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Midkine.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Midkine.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant Human Midkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 16 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
    • Sequence similarities
      Belongs to the pleiotrophin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amphiregulin associated protein antibody
      • Amphiregulin-associated protein antibody
      • ARAP antibody
      • FLJ27379 antibody
      • Mdk antibody
      • Midgestation and kidney protein antibody
      • Midkine antibody
      • MK 1 antibody
      • MK antibody
      • MK_HUMAN antibody
      • MK1 antibody
      • NEGF 2 antibody
      • NEGF2 antibody
      • Neurite growth promoting factor 2 antibody
      • Neurite outgrowth promoting protein antibody
      • Neurite outgrowth-promoting factor 2 antibody
      • Neurite outgrowth-promoting protein antibody
      • Retinoic acid inducible factor antibody
      see all

    References

    ab83160 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab83160.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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