Anti-Midkine antibody - C-terminal (ab170820)

Overview

  • Product name
    Anti-Midkine antibody - C-terminal
    See all Midkine primary antibodies
  • Description
    Rabbit polyclonal to Midkine - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Midkine aa 110-143 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P21741

  • Positive control
    • Mouse kidney tissue lysate; Human pancreas tissue; HeLa and HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab170820 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 16 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • Sequence similarities
    Belongs to the pleiotrophin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

Images

  • Anti-Midkine antibody - C-terminal (ab170820) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size: 16 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Midkine with ab170820 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal immunofluorescent analysis of HeLa cells labeling Midkine with ab170820 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Confocal immunofluorescent analysis of HepG2 cells labeling Midkine with ab170820 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

References

ab170820 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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