Overview

  • Product nameAnti-Midkine antibody [EP1143Y]
    See all Midkine primary antibodies
  • Description
    Rabbit monoclonal [EP1143Y] to Midkine
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus (Human)

  • Positive control
    • Human liver carcinoma. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab52637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/30.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.

Target

  • FunctionHas heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • Sequence similaritiesBelongs to the pleiotrophin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

Anti-Midkine antibody [EP1143Y] images

  • Human liver carcinoma stained with ab52637 at 1/100 - 1/250 dilution
  • Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution + Recombinant protein at 0.001 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • HeLa cells stained with ab52637 at 1/250 - 1/500 dilution

References for Anti-Midkine antibody [EP1143Y] (ab52637)

This product has been referenced in:
  • Yamashita T  et al. Serum midkine as a biomarker for malignancy, prognosis, and chemosensitivity in head and neck squamous cell carcinoma. Cancer Med 5:415-25 (2016). IHC-P ; Human . Read more (PubMed: 26798989) »
  • Yuan K  et al. MDK Protein Overexpression Correlates with the Malignant Status and Prognosis of Non-small Cell Lung Cancer. Arch Med Res 46:635-41 (2015). WB, IHC-P ; Human . Read more (PubMed: 26656665) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us. This product is a tissue culture supernatant, not a purified antibody. Concentrations are generally not determined for tissue culture supernatant products as they are not relevance; they may contain other proteins aside fro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"