• Product nameAnti-Midkine antibody [EP1143Y]
    See all Midkine primary antibodies
  • Description
    Rabbit monoclonal [EP1143Y] to Midkine
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus (Human)

  • Positive control
    • Human liver carcinoma. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP 1/30.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionHas heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
    • Sequence similaritiesBelongs to the pleiotrophin family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amphiregulin associated protein antibody
      • Amphiregulin-associated protein antibody
      • ARAP antibody
      • FLJ27379 antibody
      • Mdk antibody
      • Midgestation and kidney protein antibody
      • Midkine antibody
      • MK 1 antibody
      • MK antibody
      • MK_HUMAN antibody
      • MK1 antibody
      • NEGF 2 antibody
      • NEGF2 antibody
      • Neurite growth promoting factor 2 antibody
      • Neurite outgrowth promoting protein antibody
      • Neurite outgrowth-promoting factor 2 antibody
      • Neurite outgrowth-promoting protein antibody
      • Retinoic acid inducible factor antibody
      see all

    Anti-Midkine antibody [EP1143Y] images

    • Human liver carcinoma stained with ab52637 at 1/100 - 1/250 dilution
    • HeLa cells stained with ab52637 at 1/250 - 1/500 dilution
    • Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution + Recombinant protein at 0.001 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa

    References for Anti-Midkine antibody [EP1143Y] (ab52637)

    This product has been referenced in:
    • Yamashita T  et al. Serum midkine as a biomarker for malignancy, prognosis, and chemosensitivity in head and neck squamous cell carcinoma. Cancer Med 5:415-25 (2016). IHC-P ; Human . Read more (PubMed: 26798989) »
    • Yuan K  et al. MDK Protein Overexpression Correlates with the Malignant Status and Prognosis of Non-small Cell Lung Cancer. Arch Med Res 46:635-41 (2015). WB, IHC-P ; Human . Read more (PubMed: 26656665) »

    See all 8 Publications for this product

    Product Wall

    Thank you for contacting us. This product is a tissue culture supernatant, not a purified antibody. Concentrations are generally not determined for tissue culture supernatant products as they are not relevance; they may contain other proteins aside fro...

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