Overview

  • Product nameAnti-MIER1 antibody
  • Description
    Rabbit polyclonal to MIER1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: KDASRRTGDE KGVEAIPEGS HIKDNEQALY ELVKCNFDTE EALRRLRFNV, corresponding to internal sequence amino acids 253-302 of Human MIER1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab26254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • RelevanceMIER1 (previously called er1) was first isolated from Xenopus laevis embryonic cells as a novel fibroblast growth factor. Human MIER1 (hMIER1) was shown to encode a nuclear protein with an N terminal acidic transcription activation domain. The human orthologue of MIER1 displays 91% similarity to the Xenopus sequence at the amino acid level and was shown to be upregulated in breast carcinoma cell lines and tumors. Alternative splicing at the 3' end of hMIER1 produces two major isoforms, hMIER1 and hMIER1ß, which contain distinct C terminal domains. The association of hMIER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMIER1 proteins and this may have important implications for hMIER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A sensitive histone deacetylase 1 (HDAC1).
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • ER1 antibody
    • Mesoderm induction early response 1 antibody
    • Mesoderm induction early response 1 homolog (Xenopus laevis) antibody
    • MI-ER1 antibody

Anti-MIER1 antibody images

References for Anti-MIER1 antibody (ab26254)

ab26254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"