Anti-MIF antibody [2Ar3] (ab14575)


  • Product nameAnti-MIF antibody [2Ar3]
    See all MIF primary antibodies
  • Description
    Mouse monoclonal [2Ar3] to MIF
  • SpecificityRecognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    E. coli-derived recombinant full length protein (Human).

  • General notes= 10ng/mg Endotoxin. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2Ar3
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab14575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at a concentration of 4 ug/ml for direct ELISA and 2 ug/ml for capture in sandwich ELISA.

    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 13.8 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
    • Involvement in diseaseGenetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    • Sequence similaritiesBelongs to the MIF family.
    • Cellular localizationSecreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
    • Information by UniProt
    • Database links
    • Alternative names
      • GIF antibody
      • GLIF antibody
      • Glycosylation inhibiting factor antibody
      • Glycosylation-inhibiting factor antibody
      • L-dopachrome isomerase antibody
      • L-dopachrome tautomerase antibody
      • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
      • Macrophage migration inhibitory factor antibody
      • MIF antibody
      • MIF protein antibody
      • MIF_HUMAN antibody
      • MMIF antibody
      • Phenylpyruvate tautomerase antibody
      see all

    References for Anti-MIF antibody [2Ar3] (ab14575)

    ab14575 has not yet been referenced specifically in any publications.

    Product Wall

    The monoclonal antibody ab14575 was raised using human MIF, recombinant protein expressed in E. coli. The epitope has not been determined. For a sandwich ELISA, I recommend ab14575 for capture and goat polyclonal ab14574 for detection, followed by ...

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