Anti-MIF antibody (ab34644)
Key features and details
- Chicken polyclonal to MIF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgY
Overview
-
Product name
Anti-MIF antibody
See all MIF primary antibodies -
Description
Chicken polyclonal to MIF -
Host species
Chicken -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig, Cynomolgus monkey, Macaque monkey -
Immunogen
Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF
-
Positive control
- Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
IgY fraction -
Purification notes
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa). |
Target
-
Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
Involvement in disease
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
Sequence similarities
Belongs to the MIF family. -
Cellular localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
-
Database links
- Entrez Gene: 280858 Cow
- Entrez Gene: 17319 Mouse
- Entrez Gene: 397412 Pig
- Entrez Gene: 81683 Rat
- Entrez Gene: 780466 Sheep
- SwissProt: P80177 Cow
- SwissProt: Q4R549 Cynomolgus monkey
- SwissProt: P34884 Mouse
see all -
Alternative names
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
Images
-
All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution
Lane 1 : Control
Lane 2 : 100 ng of recombinant
MIF present in a bacterial lysate
Secondary
Lane 2 : Goat anti chicken at 1/5000 dilution
Predicted band size: 14 kDa
Observed band size: 12.3 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (0)
ab34644 has not yet been referenced specifically in any publications.