Overview

  • Product nameAnti-MIF antibody
    See all MIF primary antibodies
  • Description
    Rabbit polyclonal to MIF
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Rat)(E. coli-expressed).

  • Positive control
    • LPS stimulated RAW cell lysate Neuro2A (mouse neuroblastoma cell line) lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
ELISA Use at an assay dependent concentration. PubMed: 19066630
IHC-Fr Use at an assay dependent concentration.
WB 1/2000.
IP 1/500.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • Involvement in diseaseGenetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • Sequence similaritiesBelongs to the MIF family.
  • Cellular localizationSecreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • Database links
  • Alternative names
    • acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody images

  • Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate

    Performed under reducing conditions.

    MIF detected by Western Blot in LPS stimulated RAW cell lysate.

  • From a review by Arsi Rosengren.
  • ab7207 staining mouse Testis tissue sections by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with sample for 20 hours at 20°C. A biotin labelled goat polyclonal to rabbit IgG antibody, diluted 1/500 was used as the secondary.

    See Abreview

  • ab7207 staining MIF in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate EDTA buffer (pH 6.2). Samples were incubated with primary antibody (1/200 in antibody diluent) for 1 hour. An undiluted Alkaline phosphatase-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-MIF antibody (ab7207) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from normal rat serum
    Lane 2 : Whole cell lysate prepared from normal rat serum
    Lane 3 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)
    Lane 4 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Observed band size : 13 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • ab7207 used at a 1/1000 dilution as detection antibody in ELISA testing MIF in rat serum samples. Samples were blocked with 1% BSA for 2 hours at room temperature. The secondary used was an HRP conjugated donkey anti-goat (H+L) polyclonal used at a 1/200,000 dilution.

    See Abreview

  • ab7207 staining MIF in murine immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/250 in 5% goat serum/3% BSA in PBS) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

References for Anti-MIF antibody (ab7207)

This product has been referenced in:
  • Zhang YY  et al. Proteomics reveals potential non-neuronal cholinergic receptor-effectors in endothelial cells. Acta Pharmacol Sin 35:1137-49 (2014). Read more (PubMed: 25088000) »
  • Zaghloul N  et al. Overexpression of Extracellular Superoxide Dismutase Protects against Brain Injury Induced by Chronic Hypoxia. PLoS One 9:e108168 (2014). WB ; Mouse . Read more (PubMed: 25268361) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (immortalised bone marrow-derived macrophages)
Specification immortalised bone marrow-derived macrophages
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. James Harris

Verified customer

Submitted Aug 24 2012

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Serum (serum)
Specification serum
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (liver)
Specification liver
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (small intestine)
Specification small intestine
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Serum)
Loading amount 10 µg
Specification Serum
Treatment 90 minutes warm ischemia and 1h reperfusion
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization No
Blocking step Dako Protein block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

Submitted May 31 2011

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA pH6,2
Permeabilization No
Blocking step Protein Block solution Dako as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2011

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"