Overview

  • Product name
  • Description
    Rabbit polyclonal to MIF4GD
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: FVLIRDGFLL PTGLSSLAQL LLLEIIEFRA AGWKTTPAAH KYYYSEVSD, corresponding to C terminal amino acids 208-256 of Human MIF4GD

  • Positive control
    • HepG2 cell lysate, Human kidney

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

Target

Anti-MIF4GD antibody images

  • Anti-MIF4GD antibody (ab51242) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • ab51242 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded, magnification 400x.

References for Anti-MIF4GD antibody (ab51242)

ab51242 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab51242.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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