Overview

  • Product name
  • Description
    Rabbit polyclonal to MIG
  • Tested applications
    Suitable for: ELISA, Neutralising, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMIG (human Monokine induced by Interferon-Gamma)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required
WB Use 0.1µg for 100.2 cells.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml.

Target

  • Function
    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C motif chemokine 9 antibody
    • chemokine (C-X-C motif) ligand 9 antibody
    • CMK antibody
    • crg-10 antibody
    • CXCL9 antibody
    • CXCL9_HUMAN antibody
    • gamma interferon induced monokine antibody
    • Gamma-interferon-induced monokine antibody
    • HuMIG antibody
    • MIG antibody
    • monokine induced by gamma interferon antibody
    • monokine induced by interferon gamma antibody
    • Monokine induced by interferon-gamma antibody
    • SCYB9 antibody
    • Small inducible cytokine B9 antibody
    • small inducible cytokine subfamily B member 9 antibody
    • Small-inducible cytokine B9 antibody
    see all

Images

  • ab9720 staining MIG in human renal tumor with parenchyma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

References

This product has been referenced in:
  • Liu W  et al. Elevated expression of IFN-inducible CXCR3 ligands predicts poor prognosis in patients with non-metastatic clear-cell renal cell carcinoma. Oncotarget 7:13976-83 (2016). IHC . Read more (PubMed: 26910919) »
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. The product is shipped as 100 ug of lyophilized antibody. If reconstituted in 200 ul of water, the concentration will be 0.5 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if...

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Thank you for contacting us. We do not currently have any feedback from customers or lab testing regarding whether it would be possible to conjugate either of these antibodies to gold, and we therefore do not have any recommendations for a pH or conce...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil (periphery))
Specification
Tonsil (periphery)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal carcinoma)
Specification
Colorectal carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2011

Thank you for contacting us for technical support regarding ab9720. This antibody has not been tested on mouse tissue therefore we do not know what sort of banding pattern it gives with MIG of this species I'm afraid. There is 73% homology between the ...

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Unfortunately, this antibody has not been tested for IHC in frozen sections, so we can't guarantee that it will work.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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