Overview

  • Product nameAnti-MIG7 antibody
  • Description
    Rabbit polyclonal to MIG7
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to the N-terminal of Human MIG7 protein.

  • Positive control
    • MCF7 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml.

Target

  • RelevanceMIG7 is a cysteine-rich protein found in cell membranes and cytoplasm of carcinoma cells. Tumor tissue and blood from over 200 cancer patients have been shown to express MIG7, regardless of tissue origin. In contrast, it has not been detectable in tissues or blood from normal subjects. Expression of MIG7 seems to be restricted to carcinoma cells and putatively to the early placenta (Cytotrophoblast) and it's expression is important for the invasive capabilities of tumor cells. MIG7 seems to be a promising cancer cell marker for the detection of migrating and circulating carcinoma cells, the detection of occult tumor cells, and for diagnosis and disease progression. In vivo, MIG7 may be a carcinoma-specific target to potentially inhibit tumour invasion and vasculogenic mimicry, thereby preventing growth of tumors and dissemination of tumor cells.
  • Cellular localizationCell Membrane and cytoplasmic.
  • Database links
  • Alternative names
    • mig 7 antibody

Anti-MIG7 antibody images

  • Anti-MIG7 antibody (ab83494) at 1/500 dilution + MCF7 cell lysate

    Predicted band size : 23 kDa
    Observed band size : 23 kDa

References for Anti-MIG7 antibody (ab83494)

ab83494 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Sorry, there was typos it was meant to be blocking.

The antigen retrieval method used was heat mediated retrieval method. The formalin-fixed tissue were boiled in 10 mM Citrate Buffer, pH 6.0 for 10 min fo...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and I suppose the problem customer is experiencing is no staining. Please try protocol ...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for your reply.

1. What the subtype of breast cancer tissue for positive control:--Breast cancer cell line: MCF-7 or Breast Ca tissue. Unfortunately, we do not have any more specific information about the type of breast cancer of th...

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Thank you for your patience. This antibody was tested on breast cancer tissue. The brief protocol for IHC is as follows:

1) HIER in 10 mM Citrate buffer, pH6.0 for 10 min, then followed by cooling down @ RT, 20 min. (Very important to prepar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"