Anti-Milk Fat Globule 1 antibody (ab89232)

Overview

  • Product nameAnti-Milk Fat Globule 1 antibody
    See all Milk Fat Globule 1 primary antibodies
  • Description
    Mouse polyclonal to Milk Fat Globule 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Milk Fat Globule 1.

  • Positive control
    • Human pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab89232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.

Target

  • FunctionPlays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificityMammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similaritiesContains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    modifications
    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localizationMembrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BA46 antibody
    • Breast epithelial antigen BA46 antibody
    • EDIL1 antibody
    • HMFG antibody
    • hP47 antibody
    • HsT19888 antibody
    • Lactadherin antibody
    • Medin antibody
    • MFG-E8 antibody
    • MFGE8 antibody
    • MFGM antibody
    • MFGM_HUMAN antibody
    • Milk fat globule EGF factor 8 antibody
    • Milk fat globule EGF factor 8 protein antibody
    • Milk fat globule-EGF factor 8 antibody
    • O acetyl disialoganglioside synthase antibody
    • OAcGD3S antibody
    • SED1 antibody
    • SPAG10 antibody
    • Sperm associated antigen 10 antibody
    • Sperm surface protein hP47 antibody
    see all

Anti-Milk Fat Globule 1 antibody images

  • Anti-Milk Fat Globule 1 antibody (ab89232) at 1 µg/ml + Human pancreas cell lysate at 25 µg

    Predicted band size : 43 kDa
  • Anti-Milk Fat Globule 1 antibody (ab89232) at 1 µg/ml + Transfected 293T Cell Line at 25 µg

    Predicted band size : 43 kDa
    Observed band size : 42.57 kDa (why is the actual band size different from the predicted?)

References for Anti-Milk Fat Globule 1 antibody (ab89232)

ab89232 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (LCL and fibroblast)
Specification LCL and fibroblast
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 09 2013


Unfortunately, the homology between mouse and human MFGE8 is only 63.7%. We generally like to see a homology of 85% or higher before we predict that an antibody will cross react with a different species.

Below are PubMed links to citation...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"