Anti-Milk Fat Globule 1 antibody [EDM45] (Biotin) (ab79273)

Overview

  • Product nameAnti-Milk Fat Globule 1 antibody [EDM45] (Biotin)
    See all Milk Fat Globule 1 primary antibodies
  • Description
    Mouse monoclonal [EDM45] to Milk Fat Globule 1 (Biotin)
  • ConjugationBiotin
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Milk Fat Globule 1. Delipidated human Milk Fat Globule 1 membrane preparation.

  • Positive control
    • KBR3 cells; breast, colon, or lung tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab79273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P (native and denatured) (Use Protein G): Use at a concentration of 1 - 2 µg/ml for 30 minute at room temperature.
    Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    IP: Use at 2 µg/mg of lysate.
    WB: Use at a concentration of 0.5 - 1 µg/ml for 2 hours at room temperature. Predicted molecular weight: 43 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
      Medin is the main constituent of aortic medial amyloid.
    • Tissue specificityMammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
    • Sequence similaritiesContains 1 EGF-like domain.
      Contains 2 F5/8 type C domains.
    • Post-translational
      modifications
      Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
    • Cellular localizationMembrane. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BA46 antibody
      • Breast epithelial antigen BA46 antibody
      • EDIL1 antibody
      • HMFG antibody
      • hP47 antibody
      • HsT19888 antibody
      • Lactadherin antibody
      • Medin antibody
      • MFG-E8 antibody
      • MFGE8 antibody
      • MFGM antibody
      • MFGM_HUMAN antibody
      • Milk fat globule EGF factor 8 antibody
      • Milk fat globule EGF factor 8 protein antibody
      • Milk fat globule-EGF factor 8 antibody
      • O acetyl disialoganglioside synthase antibody
      • OAcGD3S antibody
      • SED1 antibody
      • SPAG10 antibody
      • Sperm associated antigen 10 antibody
      • Sperm surface protein hP47 antibody
      see all

    References for Anti-Milk Fat Globule 1 antibody [EDM45] (Biotin) (ab79273)

    ab79273 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"