The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab17787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 40-45 kDa).|
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|