Anti-Mimitin antibody - C-terminal (ab185374)

Overview

  • Product name
    Anti-Mimitin antibody - C-terminal
    See all Mimitin primary antibodies
  • Description
    Rabbit polyclonal to Mimitin - C-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Mimitin aa 85-169 (C terminal).
    Sequence:

    MEEILKNEKHREEIKIKSQDFYEKEKLLSKETSEELLPPPVQTQIKGHAS APYFGKEEPSVAPSSTGKTFQPGSWMPRDGKSHNQ


    Database link: Q8N183

  • Positive control
    • Human kidney tissue; Human cell line U-251MG sp and RT4 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab185374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a molecular chaperone for mitochondrial complex I assembly.
  • Tissue specificity
    Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts.
  • Involvement in disease
    Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • Sequence similarities
    Belongs to the complex I NDUFA12 subunit family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • B17.2 like antibody
    • B17.2-like antibody
    • B17.2L antibody
    • FLJ22398 antibody
    • MIMIT_HUMAN antibody
    • Mimitin antibody
    • Mimitin mitochondrial antibody
    • mitochondrial antibody
    • MMTN antibody
    • Myc induced mitochondrial protein antibody
    • Myc-induced mitochondrial protein antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 antibody
    • NADH dehydrogenase (ubiquinone) complex I, assembly factor 2 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 antibody
    • NDUFA12 like antibody
    • NDUFA12 like protein antibody
    • NDUFA12-like protein antibody
    • NDUFA12L antibody
    • NDUFAF2 antibody
    • OTTHUMP00000161882 antibody
    • OTTHUMP00000221703 antibody
    see all

Images

  • All lanes : Anti-Mimitin antibody - C-terminal (ab185374) at 1/500 dilution

    Lane 1 : Human cell line RT4 lysate
    Lane 2 : Human cell line U-251MG sp lysate


    Predicted band size : 20 kDa
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Mimitin using ab185374 at 1/1000 dilution.

References

ab185374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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