Overview

  • Product name
  • Description
    Rabbit polyclonal to MINA53
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 15 N terminal amino acids of Human MINA53 (NP_694822).

  • Positive control
    • Human heart tissue lysate; Mouse heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab126282 is affinity chromatography purified via peptide column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • Function
    Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • Tissue specificity
    Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • Sequence similarities
    Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • Cellular localization
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Images

  • Immunofluorescence of MINA53 in mouse heart tissue with ab126282 at 20 ug/mL.

  • Lane 1 : Anti-MINA53 antibody (ab126282) at 1 µg/ml
    Lane 2 : Anti-MINA53 antibody (ab126282) at 2 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 53 kDa
  • ab126282, at 5 ug/ml, staining MINA53 in Paraffin-embedded Mouse heart tissue by Immunohistochemistry.

References

ab126282 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsils)
Specification
Tonsils
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS/EDTA Buffer pH 9,0
Permeabilization
Yes - Wash -Buffer with Tween from Dako
Username

Mr. Rudolf Jung

Verified customer

Submitted Aug 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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