• Product nameAnti-MINA53 antibody
    See all MINA53 primary antibodies
  • Description
    Rabbit polyclonal to MINA53
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat, Pig
  • Immunogen

    Synthetic peptide: MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK , corresponding to amino acids 1-50 of Human MINA53

  • Positive control
    • HepG2 cell lysate; human intestine tissue



Our Abpromise guarantee covers the use of ab50908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 30, 45 kDa (predicted molecular weight: 32, 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.


  • FunctionInvolved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • Tissue specificityExpressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • Sequence similaritiesBelongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • Cellular localizationNucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Anti-MINA53 antibody images

  • Anti-MINA53 antibody (ab50908) at 1.25 µg/ml + HepG2 cell Lysate (10µg)

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32, 53 kDa
    Observed band size : 30,45 kDa (why is the actual band size different from the predicted?)
  • ab50908 at a concentration of 4-8µg/ml positively labelling epithelial cells of human intestinal villus(indicated by arrows) in paraffin-embedded tissue. Magnification 400X
  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-MINA antibody (ab50908)

References for Anti-MINA53 antibody (ab50908)

ab50908 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Sample Human Tissue sections (colon cancer)
Specification colon cancer
Permeabilization No
Fixative Formaldehyde

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Submitted May 13 2014