- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MIOS antibody
See all MIOS primary antibodies -
DescriptionRabbit polyclonal to MIOS
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
antigen sequence, corresponding to amino acids 620-741 of Human MIOS.
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Positive control
- Human duodenum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab122822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Sequence similaritiesBelongs to the WD repeat mio family.
Contains 6 WD repeats. - Information by UniProt
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Database links
- Entrez Gene: 54468 Human
- Omim: 615359 Human
- SwissProt: Q9NXC5 Human
- Unigene: 520215 Human
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Alternative names
- MIO_HUMAN antibody
- MIOS antibody
- Missing Oocyte, Meiosis Regulator, Homolog (Drosophila) antibody
- WD repeat-containing protein mio antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MIOS antibody (ab122822)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and cell junctions. Recommended concentration of ab122822 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOS antibody (ab122822)ab122822, at 1/200 dilution, staining MIOS in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
References
ab122822 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"