Overview

  • Product nameMIP1a (CCL3) Human ELISA KitSee all Macrophage Inflammatory Protein 1 alpha / CCL3 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 125.2 120% - 132%
    Serum 87.12 74% - 93%
    Plasma 97.15 81% - 112%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MIP1a (CCL3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1a in serum, plasma (recommended using EDTA or Heparin plasma), and cell culture supernatants.

    This assay employs an antibody specific for Human MIP1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C C motif chemokine 3
    • CCL3
    • CCL3_HUMAN
    • Chemokine (C C motif) ligand 3
    • Chemokine ligand 3
    • G0/G1 switch regulatory protein 19 1
    • G0/G1 switch regulatory protein 19-1
    • G0S19 1
    • G0S19 1 protein
    • Heparin binding chemotaxis protein
    • L2G25B
    • LD78-alpha(4-69)
    • LD78ALPHA
    • Macrophage inflammatory protein 1 alpha
    • Macrophage inflammatory protein 1-alpha
    • MIP 1 alpha
    • MIP-1-alpha
    • MIP-1-alpha(4-69)
    • MIP1A
    • PAT 464.1
    • SCYA 3
    • SCYA3
    • SIS alpha
    • SIS beta
    • SIS-beta
    • Small inducible cytokine A3
    • Small-inducible cytokine A3
    • Tonsillar lymphocyte LD78 alpha protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

MIP1a (CCL3) Human ELISA Kit images

  • Representative Standard Curve using ab100596.

Protocols

References for MIP1a (CCL3) Human ELISA Kit (ab100596)

ab100596 has not yet been referenced specifically in any publications.

Product Wall

CCL3-MIP1a human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL3-MIP1a human macrophage secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by CCL3 secretion by M1, M2a & M2c-DEX macrophages subtyes, particularly at day 7.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone.
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Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"