Overview

  • Product nameMIP1a (CCL3) Mouse ELISA KitSee all Macrophage Inflammatory Protein 1 alpha / CCL3 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.58 86% - 104%
    Serum 88.58 83% - 99%
    Plasma 87.94 82% - 98%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MIP-1a Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP-1a in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for mouse MIP-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C C motif chemokine 3
    • CCL3
    • CCL3_HUMAN
    • Chemokine (C C motif) ligand 3
    • Chemokine ligand 3
    • G0/G1 switch regulatory protein 19 1
    • G0/G1 switch regulatory protein 19-1
    • G0S19 1
    • G0S19 1 protein
    • Heparin binding chemotaxis protein
    • L2G25B
    • LD78-alpha(4-69)
    • LD78ALPHA
    • Macrophage inflammatory protein 1 alpha
    • Macrophage inflammatory protein 1-alpha
    • MIP 1 alpha
    • MIP-1-alpha
    • MIP-1-alpha(4-69)
    • MIP1A
    • PAT 464.1
    • SCYA 3
    • SCYA3
    • SIS alpha
    • SIS beta
    • SIS-beta
    • Small inducible cytokine A3
    • Small-inducible cytokine A3
    • Tonsillar lymphocyte LD78 alpha protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

MIP1a (CCL3) Mouse ELISA Kit images

  • Representative standard curve using ab100726

Protocols

References for MIP1a (CCL3) Mouse ELISA Kit (ab100726)

ab100726 has not yet been referenced specifically in any publications.

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