Overview

  • Product nameMIP3a (CCL20) Human ELISA Kit
    See all Macrophage Inflammatory Protein 3 alpha kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    0.8 pg/ml - 600 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.25 83% - 102%
    Serum 95.39 83% - 103%
    Plasma 93.88 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MIP3a Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3a in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • Tissue specificityExpressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Beta chemokine exodus 1
    • Beta-chemokine exodus-1
    • C C motif chemokine 20
    • CC chemokine LARC
    • Ccl20
    • CCL20(2-70)
    • CCL20_HUMAN
    • Chemokine (C C motif) ligand 20
    • CKb4
    • Exodus 1
    • LARC
    • Liver and activation regulated chemokine
    • Liver and activation-regulated chemokine
    • Macrophage inflammatory protein 3 alpha
    • MIP 3 alpha
    • MIP 3a
    • MIP-3-alpha
    • MIP3 alpha
    • MIP3A
    • MIP3alpha
    • SCYA20
    • Small inducible cytokine A20
    • Small inducible cytokine subfamily A (Cys Cys) member 20
    • Small-inducible cytokine A20
    • ST38
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

MIP3a (CCL20) Human ELISA Kit images

  • Representative Standard Curve using ab100599.

  • Representative Standard Curve using ab100599.

Protocols

References for MIP3a (CCL20) Human ELISA Kit (ab100599)

ab100599 has not yet been referenced specifically in any publications.

Product Wall

CCL20-MIP3a secretion by human macrophages & DC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL20-MIP3a human macrophage and DC secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by CCL3 secretion by M1 macrophages subtyes, particularly at day 3.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone DC = GMCSF+IL4.
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Submitted May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"