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Rabbit polyclonal to MIPEP
Recombinant fragment corresponding to a region within amino acids 180-396 of Human MIPEP (UniProt ID: Q99797).
- A431 and HepG2 whole cell lysates; A431 cells; Human CL15 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 81 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
||1/100 - 1/1000.
Cleaves proteins, imported into the mitochondrion, to their mature size.
Belongs to the peptidase M3 family.
Information by UniProt
- EC 126.96.36.199 antibody
- HMIP antibody
- MIP antibody
Western blot - Anti-MIPEP antibody (ab154407)
Anti-MIPEP antibody (ab154407) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (ab154407)
Immunofluorescence analysis of methanol-fixed A431 cells, labeling MIPEP using ab154407 at a 1/200 dilution.
The image in the lower panel was co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (ab154407)
Immunohistochemical analysis of paraffin embedded Human CL15 xenograft tissue, labeling MIPEP using ab154407 at a 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"