Anti-MIRO1 antibody (ab55035)

Overview

  • Product nameAnti-MIRO1 antibody
    See all MIRO1 primary antibodies
  • Description
    Mouse monoclonal to MIRO1
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MIRO1 aa 483-581.
    Sequence:

    TEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLH EVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP


    Database link: Q8IXI2

Properties

Applications

Our Abpromise guarantee covers the use of ab55035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • Tissue specificityUbiquitously expressed. Expressed at high level in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.
  • Cellular localizationMitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHT 1 antibody
    • ARHT1 antibody
    • FLJ11040 antibody
    • FLJ12633 antibody
    • hMiro 1 antibody
    • hMiro-1 antibody
    • hMiro1 antibody
    • MIRO 1 antibody
    • MIRO-1 antibody
    • MIRO1 antibody
    • MIRO1_HUMAN antibody
    • mitochondrial Rho (MIRO) GTPase 1 antibody
    • Mitochondrial Rho 1 antibody
    • Mitochondrial Rho GTPase 1 antibody
    • Mitochondrial Rho1 antibody
    • Rac GTP binding protein like protein antibody
    • Rac-GTP-binding protein-like protein antibody
    • Ras homolog family member T1 antibody
    • Ras homolog gene family member T1 antibody
    • Rhot 1 antibody
    • RHOT1 antibody
    see all

Anti-MIRO1 antibody images

  • Rhot1 antibody (ab55035) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab55035 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55035, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-MIRO1 antibody (ab55035)

ab55035 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (heart)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 16 2015

Gracias por ponerte en contacto con nosotros. En nuestro catálogo se encuentran disponibles dos anticuerpos contra la proteína Miro-1: ab55035: http://www.abcam.com/Rhot1-antibody-ab55035.html Que solo se ha testado en WB y sELISA, y reconoce la forma ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"