Synthetic peptide corresponding to Mouse MIRO2 aa 400-500 conjugated to keyhole limpet haemocyanin. Database link: Q8JZN7
This antibody gave a positive signal in the following Mouse tissues; Heart; Skeletal Muscle; Kidney; Pancreas.
This antibody gave a positive result when used in the following methanol fixed cell lines: Hek293.
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ICC/IF image of ab154946 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154946 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Anti-MIRO2 antibody (ab154946)
All lanes : Anti-MIRO2 antibody (ab154946) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Mouse) Tissue Lysate Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate Lane 3 : Kidney (Mouse) Tissue Lysate Lane 4 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154946 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406