Overview

  • Product nameAnti-MIRO2 antibody
    See all MIRO2 primary antibodies
  • Description
    Mouse polyclonal to MIRO2
  • SpecificityThis antibody has been tested with MIRO2 transfected 293T cell lysate. We have no data on endogenous samples.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human MIRO2.
    Database link: Q8IXI1

  • Positive control
    • MIRO2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa.

Target

  • FunctionMitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • Tissue specificityUbiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.
  • Cellular localizationMitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHT2 antibody
    • C16orf39 antibody
    • hMiro 2 antibody
    • hMiro-2 antibody
    • hMiro2 antibody
    • MIRO-2 antibody
    • MIRO2_HUMAN antibody
    • Mitochondrial Rho GTPase 2 antibody
    • Ras homolog gene family member T2 antibody
    • RASL antibody
    • RHOT2 antibody
    see all

Anti-MIRO2 antibody images

  • All lanes : Anti-MIRO2 antibody (ab67588) at 1/500 dilution

    Lane 1 : RHOT2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-MIRO2 antibody (ab67588)

ab67588 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for sending the image.

Please keep me updated about the results with the replacement antibody, and let me know if there is anything else that we can do for you.

Have a nice day.

Thank you for your call today and for letting us know about the trouble with ab67588. I apologize again for the previous confusion regarding the expected molecular weight of this protein.

As we discussed, I'm sending a free of charge vial of ...

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Thank you for your inquiry.

I was in touch with the lab and they confirmed that they did not test this product in endogenous samples, only RHOT2 transfected 293T cell lysate.

However, they were able to update us with a new WB imag...

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Thank you for bringing this to our attention. I've been in touch with the lab to see if they have any endogenous testing data.
Here is the subcellular fractionation protocol we talked about on the phone.
http://www.abcam.com/index.html?pageco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"