MIND kinetochore complex component, homolog (yeast) antibody
MIS12 homolog (yeast) antibody
MIS12 homolog antibody
MIS12 MIND kinetochore complex component homolog (yeast) antibody
MTW 1 antibody
Protein MIS12 homolog antibody
Anti-MIS12 antibody images
Immunoprecipitation - MIS12 antibody (ab70843)
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - MIS12 antibody (ab70843)
All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-MIS12 antibody (ab70843)This image is courtesy of an abreview by Eleni Petsalaki.
ab70843 staining MIS12 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour. An undiluted FITC-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
References for Anti-MIS12 antibody (ab70843)
This product has been referenced in:
Milev MP et al. TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. J Cell Biol209:221-34 (2015).
Read more (PubMed: 25918224) »
Petrovic A et al. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. J Cell Biol190:835-52 (2010).
Read more (PubMed: 20819937) »