Overview

  • Product nameAnti-MiTF antibody [21D1418]
    See all MiTF primary antibodies
  • Description
    Mouse monoclonal [21D1418] to MiTF
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Quail
  • Immunogen

    Synthetic peptide:

    SEARALAKERQK

    , corresponding to amino acids 301-312 of Human MiTF.

  • Positive control
    • A375 (human) and Raw (mouse) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
  • Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
  • Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
    Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
    Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
  • Sequence similaritiesBelongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHLHE32 antibody
    • bHLHe32 antibody
    • Class E basic helix-loop-helix protein 32 antibody
    • CMM8 antibody
    • Homolog of mouse microphthalmia antibody
    • Mi antibody
    • Microphthalmia associated transcription factor antibody
    • Microphthalmia, mouse, homolog of antibody
    • Microphthalmia-associated transcription factor antibody
    • MITF antibody
    • MITF_HUMAN antibody
    • mitfa antibody
    • nacre antibody
    • WS2 antibody
    • WS2A antibody
    • z3A.1 antibody
    see all

Anti-MiTF antibody [21D1418] images



  • Predicted band size : 59 kDa


    Western blot using MiTF antibody [21D1418] (ab13703)

    Lane 1: A375 cell lysate (human) using 1:1000 dilution
    Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution

    Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique

  • Overlay histogram showing Malme-3 cells stained with ab13703 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13703, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-MiTF antibody [21D1418] (ab13703)

This product has been referenced in:
  • Liang H  et al. CtBP2 Downregulation during Neural Crest Specification Induces Expression of Mitf and REST, Resulting in Melanocyte Differentiation and Sympathoadrenal Lineage Suppression. Mol Cell Biol 31:955-70 (2011). WB ; Quail . Read more (PubMed: 21199918) »
  • Shimoda N  et al. Effect of heterocyclic pyrimidine compounds on UVB-induced cell damage in human keratinocytes and on melanogenesis in mouse B16 cells. Biol Pharm Bull 33:862-8 (2010). WB ; Mouse . Read more (PubMed: 20460767) »

See all 4 Publications for this product

Product Wall

I'm very sorry to hear you are experiencing problems with ab13703 and for the unexpected delay in the production of the C5 clone. I understand you are disappointed, you mention controls, are these nuclear lysates of A375 or Raw cells? These are our...

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I'm very sorry to hear you are having problems with ab13703, thank you for providing your protocol details, these are very useful. I would like to recommend the following modifications to your protocol: -I would not recommend using DTT in the lys...

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Thank you for your e-mail. I have placed a new order for you one vial of ab12039 - free of charge. Please do let us know how you are getting on with this alternative antibody. Good luck with your research!

Thank you for your e-mail and for taking your time to fill in the on-line Questionnaire. I am very sorry to hear that you are having problem with this antibody. I would be grateful if you could give me the batch number you have tested. 1. Have ...

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I have received the following information from the source of ab13703: "We reviewed the QC data for this antibody and it is very clean in our hands. QC data for MCF7, T98G, Daudi, A375, and Raw all reveal 1 clean single band at the correct molecular...

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My apologies for the problem and the delay in replying to you, we have been trying to contact the source of ab13703 to find out if they have some suggestions and unfortunately we are still waiting to hear from them. Would you please let me know if ...

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Thank you for your enquiry. We don't have this particular cell positive control within our catalogue. In the future if you cannot find what you are looking for within the Abcam catalogue you can click on the link to the "World's antibody gateway". The ...

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Thank you for your enquiry. It has been tested only in A375 and Raw cells. Here is the sequence information:CPSTGLSBPDLVN. When I did a BLAST search I found it matched the MITF-L/MITF-A isoform, so I would expect it to cross react with MITF-L, alth...

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