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SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF.
Our Abpromise guarantee covers the use of ab13703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Western blot using MiTF antibody [21D1418] (ab13703)
Lane 1: A375 cell lysate (human) using 1:1000 dilution
Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution
Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique