Overview

  • Product name
  • Description
    Rabbit polyclonal to MiTF
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: IHC (PFA fixed)
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human MiTF.

    (Peptide available as ab21454.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) A431 (Human epithelial carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in HeLa nuclear lysate This antibody gave a positive signal in the following tissue lysates: Brain (Mouse and Rat) Testis (Mouse) Spinal Cord (Mouse) Ovary (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab20663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22131883
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for IHC (PFA fixed).
  • Target

    • Function
      Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
    • Tissue specificity
      Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
    • Involvement in disease
      Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
      Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
      Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
    • Sequence similarities
      Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BHLHE32 antibody
      • bHLHe32 antibody
      • Class E basic helix-loop-helix protein 32 antibody
      • CMM8 antibody
      • Homolog of mouse microphthalmia antibody
      • Mi antibody
      • Microphthalmia associated transcription factor antibody
      • Microphthalmia, mouse, homolog of antibody
      • Microphthalmia-associated transcription factor antibody
      • MITF antibody
      • MITF_HUMAN antibody
      • mitfa antibody
      • nacre antibody
      • WS2 antibody
      • WS2A antibody
      • z3A.1 antibody
      see all

    Anti-MiTF antibody images

    • All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa nuclear lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : HeLa whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/ml
      Lane 5 : HeLa nuclear lysate with Human MiTF peptide (ab21454) at 1 µg/ml
      Lane 6 : A431 whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
      Additional bands at : 25 kDa,37 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml

      Lane 1 : NIH 3T3 whole cell lysate (ab7179)
      Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate
      Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
      Lane 5 : Spinal Cord (Mouse) Tissue Lysate
      Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
      Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
      Lane 8 : Brain (Rat) Tissue Lysate - normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 52 kDa
      Observed band size : 52 kDa

    References for Anti-MiTF antibody (ab20663)

    This product has been referenced in:
    • Acosta-Andrade C  et al. A novel role for antizyme inhibitor 2 as a regulator of serotonin and histamine biosynthesis and content in mouse mast cells. Amino Acids 48:2411-21 (2016). Read more (PubMed: 27084713) »
    • Allouche J  et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Flow Cyt ; Human . Read more (PubMed: 26150484) »

    See all 6 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Sample
    Mouse Tissue sections (skin tumor (Melanoma))
    Specification
    skin tumor (Melanoma)
    Permeabilization
    Yes - PBS with Triton-X 100
    Fixative
    Paraformaldehyde
    Username

    Otataren

    Verified customer

    Submitted Feb 25 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Sample
    Human Tissue sections (melanoma)
    Specification
    melanoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jun 10 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Sample
    Mouse Tissue sections (skin)
    Specification
    skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 07 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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