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Recombinant fragment corresponding to Human MiTF (N terminal).
Our Abpromise guarantee covers the use of ab12039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 16648630
Fix with methanol/acetone, block with 1% BSA/PBS (see Wellbrock et al).
|ChIP||Use at an assay dependent concentration. PubMed: 21990371|
|EMSA||Use at an assay dependent concentration.|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).|
|IP||Use at 2 µg/mg of lysate.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"