The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300 - 1/1000.
Use at an assay dependent concentration.
1/5000. Predicted molecular weight: 58 kDa.
Different splicing isoforms detected
FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance. Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness. Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Sequence similaritiesBelongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8 μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody.
At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).
References for Anti-MiTF antibody - ChIP Grade (ab122982)
This product has been referenced in:
Tsukiji N et al. Mitf functions as an in ovo regulator for cell differentiation and proliferation during development of the chick RPE. Dev Biol326:335-46 (2009).
Read more (PubMed: 19100253) »
Delmas V et al. Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development. Genes Dev21:2923-35 (2007).
Read more (PubMed: 18006687) »