Overview

  • Product nameAnti-MiTF antibody - ChIP Grade
    See all MiTF primary antibodies
  • Description
    Rabbit polyclonal to MiTF - ChIP Grade
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Xenopus laevis
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human MiTF.
    Database link: O75030

  • Positive control
    • Chicken embryo.

Properties

Applications

Our Abpromise guarantee covers the use of ab122982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/300.
WB 1/5000. Predicted molecular weight: 58 kDa.

Different splicing isoforms detected

ChIP 1/200.

Target

  • FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
  • Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
  • Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
    Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
    Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
  • Sequence similaritiesBelongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BHLHE32 antibody
    • bHLHe32 antibody
    • Class E basic helix-loop-helix protein 32 antibody
    • CMM8 antibody
    • Homolog of mouse microphthalmia antibody
    • Mi antibody
    • Microphthalmia associated transcription factor antibody
    • Microphthalmia, mouse, homolog of antibody
    • Microphthalmia-associated transcription factor antibody
    • MITF antibody
    • MITF_HUMAN antibody
    • mitfa antibody
    • nacre antibody
    • WS2 antibody
    • WS2A antibody
    • z3A.1 antibody
    see all

Anti-MiTF antibody - ChIP Grade images

  • Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8
    μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody. 

    At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).

  • Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).

References for Anti-MiTF antibody - ChIP Grade (ab122982)

This product has been referenced in:
  • Tsukiji N  et al. Mitf functions as an in ovo regulator for cell differentiation and proliferation during development of the chick RPE. Dev Biol 326:335-46 (2009). Read more (PubMed: 19100253) »
  • Delmas V  et al. Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development. Genes Dev 21:2923-35 (2007). Read more (PubMed: 18006687) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"