Overview

  • Product name
    Anti-MiTF (phospho S73) antibody
    See all MiTF primary antibodies
  • Description
    Rabbit polyclonal to MiTF (phospho S73)
  • Specificity
    Detects endogenous levels of MiTF only when phosphorylated at serine 73.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of serine 73 (PNSPPM)

  • Positive control
    • Cos 7 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab59201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
    • Tissue specificity
      Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
    • Involvement in disease
      Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
      Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
      Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
    • Sequence similarities
      Belongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BHLHE32 antibody
      • bHLHe32 antibody
      • Class E basic helix-loop-helix protein 32 antibody
      • CMM8 antibody
      • Homolog of mouse microphthalmia antibody
      • Mi antibody
      • Microphthalmia associated transcription factor antibody
      • Microphthalmia, mouse, homolog of antibody
      • Microphthalmia-associated transcription factor antibody
      • MITF antibody
      • MITF_HUMAN antibody
      • mitfa antibody
      • nacre antibody
      • WS2 antibody
      • WS2A antibody
      • z3A.1 antibody
      see all

    Images

    • All lanes : Anti-MiTF (phospho S73) antibody (ab59201) at 1/500 dilution

      Lane 1 : Cos 7 cell extract
      Lane 2 : Cos 7 cell extract with phosphopeptide


      Predicted band size : 59 kDa
      Observed band size : 59 kDa

    References

    ab59201 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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