Anti-Mitochondria antibody [113-1] (ab92824)

Overview

  • Product name
    Anti-Mitochondria antibody [113-1]
    See all Mitochondria primary antibodies
  • Description
    Mouse monoclonal [113-1] to Mitochondria
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Human cell homogenate

  • Positive control
    • WB: HeLa cell lysate
  • General notes


    This antibody is an excellent marker for Human cells in xenographic model research.

Properties

Applications

Our Abpromise guarantee covers the use of ab92824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa.
IHC-P 1/1000.
ICC/IF 1/800.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • Cellular localization
    Mitochondrial

Images

  • Anti-Mitochondria antibody [113-1] (ab92824) at 1/1000 dilution + HeLa lysate

    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab92824 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab92824 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92824, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab92824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92824, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab92824 staining mitochondria in the Human U87 glioblastoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% NBF, permeabilized with Triton X-100 and blocked with 2% serum for 90 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in 0.2% BSA + 2% NGS) for 15 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG1 polyclonal was used as the secondary antibody (1/500). Co stained with ActinGreen, 2 drops/ml PBS, 10 min and Hoechst 2ug/ml in H2O 10 min

    See Abreview

References

This product has been referenced in:
  • Lee J  et al. Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells. Nat Commun 8:14686 (2017). Read more (PubMed: 28272465) »
  • Eyre R  et al. Patient-derived Mammosphere and Xenograft Tumour Initiation Correlates with Progression to Metastasis. J Mammary Gland Biol Neoplasia 21:99-109 (2016). ICC ; Human . Read more (PubMed: 27680982) »

See all 11 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human cells in rat heart xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Specification
Human cells in rat heart xenograft
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Srishti Bhutani

Verified customer

Submitted Feb 02 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
skin tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (RAB-9 skin Fibroblast)
Permeabilization
Yes - 0.25% Triton X-100
Specification
RAB-9 skin Fibroblast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Christian Elabd

Verified customer

Submitted Feb 19 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87 glioblastoma)
Permeabilization
Yes - triton x 100
Specification
U87 glioblastoma
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% NBF, 10 min
Username

Virginia Hoglund

Verified customer

Submitted Jul 27 2015

Thank you for contacting us.

Unfortunately we will not be able to provide this antibody conjugated with Biotin or any other conjugate. Could you however let us know if we can provide further help in your search e.g. if you are trying to look f...

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I have a further update relating to your enquiry.

The Anti-Mitochondria antibody [113-1], ab92824, should also be suitable for the customers experiments. It has been shown not to cross react with either mouse or rat. Characterization in both W...

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Thank you for contacting us and your interest in our products.

As discussed over the phone, the anti-actin antibody ab3280 has been shown to reacts with all six known vertebrate isoforms of actin. It also reacts with two cytoplasmic actins (be...

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I apologize for the delay; the concentration of lot GR29268-9 is 1 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for contacting us.

We do have mitochondrial markers which will only recognize human cells and therefore used in xenographs.

Anti-Mitochondria antibody [113-1] (ab92824) is tested inWB,IHC-P,IHC-Fr,ICC/IF, does not reac...

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Thank you for contacting us.

All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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