Semi purified mitochondrial preparation.
Our Abpromise guarantee covers the use of ab3298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent dilution. Used at a dilution of 1/50 for 2 hours (see Abreview for further information).|
|ICC||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. PubMed: 18167556Samples require boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min. This antibody can be used to stain themitochondria in cell / tissue preparations and can be used as a marker of the mitochondria in subcellular fractions.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 2 µg/ml. This antibody detects a band of 60 kDa, which corresponds to the predicted molecular weight of the non glycosylated protein component of mitochondria.|
|Flow Cyt||Use 1µg for 106 cells.|
Mitochondria were detected by Immunofluorescence in U373 human glioblastoma cells using Mitochondrial Marker antibody (ab3298).
ab3298 at 0.2µg/ml staining Mitochondria-Mitochondrial Marker from human cardiac muscle sections by Immunohistochemistry (Paraffin-embedded sections). The antibody was incubated with the tissue for 2 hours and then detected with an Alexa-Fluor® 488 conjugated anti-mouse IgG secondary antibody.
This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.
ab3298 staining HEK 293 cells by ICC/IF. Following paraformaldehyde fixation and permeabilisation with 0.5%TX100 the cells were blocked with 5% serum (for 20 hours at 25°C). The primary antibody was diluted 1/100 in PBS, 2% goat serum, 0.1% TX100 and incubated for 1 hour at 25°C. An Alexa Fluor® 546 conjugated goat anti-mouse was used as the secondary.
Mitochondria are labeled with red fluorescence, and the nuclei were counterstained with DAPI.