Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)

Overview

  • Product nameAnti-Mitochondria antibody [MTC02] (Biotin)
    See all Mitochondria primary antibodies
  • Description
    Mouse monoclonal [MTC02] to Mitochondria (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Semi-purified mitochondrial preparation.

  • Positive control
    • HeLa cells. Liver.
  • General notes


    ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceMitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
  • Cellular localizationMitochondrial

Anti-Mitochondria antibody [MTC02] (Biotin) images

  • ab79479 staining Mitochondria in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of ab79479 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)

ab79479 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Ventana Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample Human Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative 10% NBF
Username

Abcam user community

Verified customer

Submitted Dec 16 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney and Spleen)
Specification Kidney and Spleen
Fixative 10% NBF
Antigen retrieval step Other
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2013

Thank you for your enquiry and interest. As the datasheets indicate these antibodies have been tested for cross-reaction with human but not data available for mouse. All of these three antibodies are raised in mouse, so high non-specific background cou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"