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Semi-purified mitochondrial preparation.
ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.
Our Abpromise guarantee covers the use of ab79479 in the following tested applications.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent dilution.|
ab79479 staining Mitochondria in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
ab79479 has not yet been referenced specifically in any publications.