Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)

Overview

  • Product name
    Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
    See all Mitochondria Isolation kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Human, a wide range of other species
  • Product overview

    ab110171 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

     

     

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab110171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. Mitochondria isolations from cultured cells.

Images

  • Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

Protocols

References

This product has been referenced in:
  • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . Read more (PubMed: 28064117) »
  • Wang K  et al. TNF-a promotes extracellular vesicle release in mouse astrocytes through glutaminase. J Neuroinflammation 14:87 (2017). Read more (PubMed: 28427419) »

See all 11 Publications for this product

Customer reviews and Q&As

1. Yes, the kit isolates intact mitochondria
2. We recommend lysing the mitochondria for IP using lauryl maltoside:
10% lauryl maltoside: ab109857, http://www.abcam.com/10-lauryl-maltoside-solution-ab109857.html
15% lauryl maltoside: ab1098...

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Reagent C of ab110171 can be frozen. This may extend the shelf-life of the reagent, particularly if it has been opened and a bacterial growth is of concern. Since we do not provide the protease inhibitors, we do not know how stable the protease inhibit...

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Thank you for contacting me. You are welcome with any questions.

I double checked with the laboratory. Reagent A contains no protein. It is a buffered solution containing sucrose and optimised detergents. Regrettably the exact contents it prop...

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Thank you for contacting us.

The homogenizer is borosilicate glass and is reusable. Below is a link to a full description and picture:


https://us.vwr.com/store/catalog/product.jsp?catalog_number=KT885300-0002


...

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Thank you for contacting us.

Samples may be prepared as purified mitochondria, crudemitochondria. In most cases whole tissue or cell lysates aresuitable but these may require some sample optimization. Mitochondria samples can be prepared bys...

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Thanks for your enquiry.

We have two kits that are designed to isolate mitochondria:

1. ab110168 - Mitochondrial isolation kit for tissues
2. ab110171 - Mitochondrial isolation kit for cultured cells

You can check t...

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Thank you for contacting us and reporting the problems this customer has been having with the Mitochondria Isolation Kit for Cultured Cells kits (ab110170 and ab110171).

As mentioned, I have consulted with the lab in MitoSciences to get their...

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Thank you for your enquiry.

The Dounce Homogenizer in this kit is made of glass and includes 2 pestles, and one 2ml column. I have included an image for your reference.

If there is anything else that I can help you with please do ...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding ab110171.

The lab used the following procedure to run the mitochondrial fraction on a Western blot:

1. Measure the protein con...

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If you are planning to use our activity kits to measure complex I activity,there is no needto keep the mitochondria viable. Whole mito preparations may be frozen at -80C and thawed at a later stage for enzyme activity. Howeverfor respiration experiment...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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