• Product nameMitochondria/Cytosol Fractionation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    4h 00m
  • Product overview

    Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • RelevanceMitochondria/Cytosol Fractionation is the process for effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of mammalian cells.


Our Abpromise guarantee covers the use of ab65320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


References for Mitochondria/Cytosol Fractionation Kit (ab65320)

This product has been referenced in:
  • Kim GT  et al. Quercetin regulates the sestrin 2-AMPK-p38 MAPK signaling pathway and induces apoptosis by increasing the generation of intracellular ROS in a p53-independent manner. Int J Mol Med 33:863-9 (2014). Read more (PubMed: 24535669) »
  • Qin Y  et al. Forkhead box L1 is frequently downregulated in gallbladder cancer and inhibits cell growth through apoptosis induction by mitochondrial dysfunction. PLoS One 9:e102084 (2014). Read more (PubMed: 25010679) »

See all 4 Publications for this product

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We do not recommend freezing the buffers after first thaw for best results. Some components can precipitate out upon freezing.

ab109719 is not suitable for this purpose, this kit is based on detergent fractionation of cellular proteins into three fractions. RNA distribution was not tested with this kit.

Mitochondrial isolation kits (ab110168 – ab1101...

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I have been in contact with the lab who have confirmed that fresh tissue can be used with this kit. For this sample type, you need to isolate out the cells from the tissues by enzymatic breakdown and follow the protocol mentioned on the datasheet ...

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You would probably be best suited using a kit that is able to isolate the membrane fraction completely if you are looking at a membrane protein like Prostaglandin E Receptor EP4. You may want to use a kit like our ab...

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The supernatant after centrifugation will be mostly cell debris including parts of the membrane. This kit is sourced from an outside lab and they consider the components of the buffers to be proprietary so we do ...

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I have been in contact with the lab regarding your inquiry. For each 10 mg of tissue, use 1 ml of the 1X Cytosolic Extraction Buffer containing DTT and Protease Inhibitors for homogenization. Follow the protoco...

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I can confirm that both kits can be used for mammalian cells (including mouse samples).

If you need any further assistance in the future, please do not hesitate to contac...

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http://www.abcam.com/ab109719 - Cell Fractionation Kit Standard: allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Unl...

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Please do not use a sonicatorin order to destroy your cells, as this will result in a complete lysis of the mitochondria as well. This means you will not be able to analyse the sample.

However, we do have a kit in which we deliv...

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Unfortunately we do not have any data regarding the use of the kit ab65320with samples homogenised with a polytron. We recommend the use of a Dounce homogenizer. Excessive homogenization should be avoided, as it can cause damage to the mitochondri...

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