Abcam's Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.
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|Components||100 tests||25 tests|
|5X Cytosol Extraction Buffer||1 x 20ml||1 x 5ml|
|DTT||1 x 110µl||1 x 110µl|
|Mitochondria Extraction Buffer||1 x 10ml||1 x 2.5ml|
|Protease Inhibitor Cocktail||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab65320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|