ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:
acetaldehyde + NAD+ -> acid + NADH
The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1cm-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.
Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C. This kit is stable for 6 months from receipt.
|Components||1 x 96 tests|
|100X acetaldehyde (2.5 M)||1 x 0.25ml|
|100X Coupler||1 x 0.25ml|
|100X NAD+||1 x 0.25ml|
|100X Reagent Dye||1 x 0.25ml|
|10X Blocking Buffer||1 x 6ml|
|1X Base Buffer||1 x 24ml|
|20X Buffer||1 x 20ml|
|96 well microplate (12 strips)||1 unit|
|Extraction Buffer||1 x 15ml|
Our Abpromise guarantee covers the use of ab115348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|