Anti-Mitochondrial Ferritin antibody (ab93428)
Key features and details
- Rabbit polyclonal to Mitochondrial Ferritin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Mitochondrial Ferritin antibody
See all Mitochondrial Ferritin primary antibodies -
Description
Rabbit polyclonal to Mitochondrial Ferritin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. -
Tissue specificity
Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 94033 Human
- Omim: 608847 Human
- SwissProt: Q8N4E7 Human
- Unigene: 105324 Human
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Alternative names
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
see all
Images
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All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Exposure time: 4 minutesBlocking buffer: 2% BSA
Gel type: MES
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab93428 has been referenced in 1 publication.
- Raha AA et al. Interplay of Ferritin Accumulation and Ferroportin Loss in Ageing Brain: Implication for Protein Aggregation in Down Syndrome Dementia, Alzheimer's, and Parkinson's Diseases. Int J Mol Sci 23:N/A (2022). PubMed: 35162984