Anti-Mitochondrial Ferritin antibody (ab93428)

Overview

  • Product name
    Anti-Mitochondrial Ferritin antibody
    See all Mitochondrial Ferritin primary antibodies
  • Description
    Rabbit polyclonal to Mitochondrial Ferritin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N4E7
    (Peptide available as ab93799)

  • Positive control
    • This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab93428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).

Target

  • Function
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
  • Tissue specificity
    Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
  • Sequence similarities
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • FerH antibody
    • Ferritin antibody
    • Ferritin H subunit antibody
    • Ferritin, mitochondrial antibody
    • Fth 3 antibody
    • Fth3 antibody
    • FTMT antibody
    • FTMT_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ferritin antibody
    • MTF antibody
    • OTTHUMP00000159158 antibody
    see all

Images

  • All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutesThe band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.

References

ab93428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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