Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444)

Overview

  • Product name
    Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody
    See all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies
  • Description
    Rabbit polyclonal to Mitochondrial Pyruvate dehydrogenase kinase 1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide derived from the sequence of human PDK1, conjugated to KLH

  • Positive control
    • WB: HeLa, rat brain, mouse brain, Vero, ESK-4, RK-13 or MDCK cytoplasmic or membrane lysates. IHC-P: human colon and hepatoma tissue sections.

Applications

Our Abpromise guarantee covers the use of ab90444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 49 kDa.
IHC-P Use at an assay dependent concentration.

Target

Images

  • IHC image of Mitochondrial Pyruvate dehydrogenase kinase 1 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Vero cell lysate
    Lane 5 : ESK-4 cell lysate
    Lane 6 : RK-13 cell lysate
    Lane 7 : MDCK cell lysate


    Predicted band size : 49 kDa

References

This product has been referenced in:
  • Gudiksen A  et al. Lack of Skeletal Muscle IL-6 Affects Pyruvate Dehydrogenase Activity at Rest and during Prolonged Exercise. PLoS One 11:e0156460 (2016). WB . Read more (PubMed: 27327080) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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