IHC image of Mitochondrial Pyruvate dehydrogenase kinase 1 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - PDK1 antibody (ab90444)
All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Rat brain lysate Lane 3 : Mouse brain lysate Lane 4 : Vero cell lysate Lane 5 : ESK-4 cell lysate Lane 6 : RK-13 cell lysate Lane 7 : MDCK cell lysate
Predicted band size : 49 kDa
References for Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444)
has not yet been referenced specifically in any publications.
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