Validated using a knockout cell line
Recombinant
RabMAb

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13015] - C-terminal (ab180171)

Overview

  • Product name
    Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13015] - C-terminal
    See all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13015] to Mitochondrial Pyruvate dehydrogenase kinase 1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Mitochondrial Pyruvate dehydrogenase kinase 1 aa 400 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15118

  • Positive control
    • Fetal heart, LNCaP and HepG2 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
    • Tissue specificity
      Expressed predominantly in the heart.
    • Sequence similarities
      Belongs to the PDK/BCKDK protein kinase family.
      Contains 1 histidine kinase domain.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
      • HGNC:8809 antibody
      • Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
      • PDH kinase 1 antibody
      • Pdk1 antibody
      • PDK1_HUMAN antibody
      • Pyruvate dehydrogenase kinase isoform 1 antibody
      • Pyruvate dehydrogenase kinase, isoenzyme 1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Human fetal heart tissue lysate (20 µg)
      Lane 4: LnCaP cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab180171 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab180171 was shown to specifically react with Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab180171 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [EPR13015] - C-terminal (ab180171) at 1/10000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa

    References

    This product has been referenced in:
    • Del Rey MJ  et al. Hif-1a Knockdown Reduces Glycolytic Metabolism and Induces Cell Death of Human Synovial Fibroblasts Under Normoxic Conditions. Sci Rep 7:3644 (2017). WB ; Human . Read more (PubMed: 28623342) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180171.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up