Mitochondrial Staining Kit - Green Fluorescence - Cytopainter (ab112143)

Overview

  • Product name
    Mitochondrial Staining Kit - Green Fluorescence - Cytopainter
    See all Mitochondria kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Mitochondrial Staining Kit - Green Fluorescence | Cytopainter (ab112143) is designed to label mitochondria in live cells with green fluorescence at Ex/Em = 490/520 nm. The kit uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.


    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

  • Notes

    Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signalling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • CytoPainter Mitochondrial Staining Kit - Green Fluorescence (ab112143). HeLa cells were incubated for 60 minutes at 37C with ab112143. The staining solution was replaced with 4%PFA for 10 minutes before a final wash in PBS prior to mounting slides. Images obtained with an Olympus BX61 microscope using a FITC filter (495/519 nm) - 1750ms exposure.

  • CytoPainter Mitochondrial Staining Kit - Green Fluorescence (ab112143). Image of U2OS cells stained with ab112143 CytoPainter Mitochondrial Staining Kit - Green Fluorescence in a black 96-well plate.

Protocols

References

This product has been referenced in:
  • Luo W  et al. Subcellular location and photodynamic therapeutic effect of chlorin e6 in the human tongue squamous cell cancer Tca8113 cell line. Oncol Lett 9:551-556 (2015). Read more (PubMed: 25621023) »

See 1 Publication for this product

Customer reviews and Q&As

1) Most likely not, it is for live cell mitochondria staining.
2) We don't have experiences on Drosophila mitochondria, it should work theoretically.

Sorry for the delay in getting back to you.

As your customer has commented, the live cell mitochondria stain, will also stain the mitochondria of dead cells. The product ab112145 uses a red fluorescent stain for this and will decrease the fluo...

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Thank you for your call today and for your enquiry.

As we discussed, I would recommend either ab14744 or ab15895 for immunostaining of mitochondria in mouse cells-

http://www.abcam.com/COX-IV-antibody-20E8C12-Mitochondrial-Loading-C...

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Thank you for contacting Abcam.

After you have labeled the live cells with the Cytopainter kit, you can then fix the cells using formaldehyde.

Please let me know if there is anything else I can help you with.

Thank you for your enquiry. The mitochondria dye for ab112143 is for live cells, and can't used after the cells were fixed.  However, our ab112143 kit is fixable (meaning that it labels the live cells, and then the cells can be fixed by formaldehyde...

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Thank you for your enquiry. I am sorry to confirm this kit has not been tested on live cells. We would suggest that incubation with ab112143 for a few hours should be ok, but not for a few days. However, regrettably we are not able to guarantee this as...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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