Anti-Mitocondrial Translational Initiation Factor 3 antibody (ab67540)

Overview

  • Product nameAnti-Mitocondrial Translational Initiation Factor 3 antibody
    See all Mitocondrial Translational Initiation Factor 3 primary antibodies
  • Description
    Mouse polyclonal to Mitocondrial Translational Initiation Factor 3
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Mitochondrial Translational Initiation Factor 3 protein (Human)

  • Positive control
    • Mitochondrial Translational Initiation Factor 3 transfected 293T cell lysate and human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.
    • Sequence similaritiesBelongs to the IF-3 family.
    • Cellular localizationMitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • DC38 antibody
      • IF 3 antibody
      • IF 3Mt antibody
      • IF-3(Mt) antibody
      • IF-3Mt antibody
      • IF3 antibody
      • IF3(mt) antibody
      • IF3M_HUMAN antibody
      • IF3mt antibody
      • mitochondrial antibody
      • Mt antibody
      • MTIF3 antibody
      • Translation initiation factor IF 3, mitochondrial precursor antibody
      • Translation initiation factor IF-3 antibody
      see all

    Anti-Mitocondrial Translational Initiation Factor 3 antibody images

    • All lanes : Anti-Mitocondrial Translational Initiation Factor 3 antibody (ab67540) at 1/500 dilution

      Lane 1 : Mitochondrial Translational Initiation Factor 3 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 32 kDa
      Observed band size : 32 kDa
    • Anti-Mitocondrial Translational Initiation Factor 3 antibody (ab67540) at 1/500 dilution + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 32 kDa
      Observed band size : 32 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Mitocondrial Translational Initiation Factor 3 antibody (ab67540)

    ab67540 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"