Overview

  • Product name
  • Description
    Rabbit polyclonal to Mitoferrin1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 309-338 of Human Mitoferrin1

  • Positive control
    • MDA-MB231 cell line, Human Brain Tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab102959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance
    Mitoferrin1, SLC25A37, belongs to the mitochondrial carrier family and contains 3 Solcar repeats. It is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells and plays an essential role in heme biosynthesis.
  • Cellular localization
    Mitochondrial inner membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • HT015 antibody
    • MFRN antibody
    • Mitochondrial iron transporter 1 antibody
    • Mitochondrial solute carrier protein antibody
    • Mitoferrin 1 antibody
    • MSC antibody
    • MSCP antibody
    • predicted protein of HQ2217 antibody
    • PRO1278 antibody
    • PRO1584 antibody
    • PRO2217 antibody
    • Solute carrier family 25 member 37 antibody
    see all

Images

  • ICC/IF image of ab102959 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102959 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Mitoferrin1 antibody (ab102959) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 37 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab102959 at a dilution of 1/50.

References

ab102959 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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